Identification of murine cytotoxic T-lymphocyte epitopes of bovine herpesvirus 1
Identifieur interne : 001937 ( Main/Exploration ); précédent : 001936; suivant : 001938Identification of murine cytotoxic T-lymphocyte epitopes of bovine herpesvirus 1
Auteurs : Douglas S. Zatechka Jr. [États-Unis] ; Nagendra R. Hegde [États-Unis] ; Kandasamy Hariharan [États-Unis] ; S. Srikumaran [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 1999.
English descriptors
- Teeft :
- Adjuvant, Allele, Aspms, Assay, Bovine, Bovine herpesvirus, Cell epitopes, Ctls, Cytotoxic, Cytotoxicity assay, Epitope, Error bars, Glycoprotein, Herpesvirus, Immunization, Immunol, Individual peptides, Lymphocyte, Lysed, Lysis, Maximum release, Molecule, Murine, Native peptides, Particular class, Peptide, Peptide motif, Peptide motifs, Recombinant, Spontaneous release, Standard deviations, Syngeneic, Synthetic peptides, Target cells, Vaccine, Viral, Viral immunol, Viral peptides, Virol, Zatechka.
Abstract
Abstract: Major histocompatibility complex (MHC) class I molecules present endogenously derived viral peptides to CD8+ cytotoxic T-lymphocytes (CTLs). The objective of this study was to identify the H-2Dd- and H-2Kd-restricted CTL epitopes of bovine herpesvirus 1 (BHV-1), based on the allele-specific peptide motifs (ASPMs) of the above class I molecules. Nine sequences conforming to the H-2Dd and H-2Kd ASPMs were identified on BHV-1 proteins, and the respective peptides were synthesized. Five of these peptides exhibited moderate to strong binding to the Dd molecule. CTLs generated by BALB/c mice immunized with BHV-1 proteins emulsified in a suitable adjuvant effectively lysed peptide-pulsed syngeneic targets, indicating that these epitopes were generated in vivo. Mice immunized with these peptides emulsified in a suitable adjuvant also developed anti-BHV-1 CTLs. These CTLs identified three veritable CTL epitopes among the “potential epitopes” synthesized based on the ASPMs. The elucidation of the CTL epitopes of BHV-1 should aid in the development of efficacious vaccines against this virus.
Url:
DOI: 10.1016/S0264-410X(98)00251-5
Affiliations:
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Zatechka Jr.</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Fair Street and East Campus Loop, Lincoln, NE 68583-0905</wicri:regionArea><placeName><region type="state">Nebraska</region></placeName></affiliation></author><author><name sortKey="Hegde, Nagendra R" sort="Hegde, Nagendra R" uniqKey="Hegde N" first="Nagendra R." last="Hegde">Nagendra R. Hegde</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Fair Street and East Campus Loop, Lincoln, NE 68583-0905</wicri:regionArea><placeName><region type="state">Nebraska</region></placeName></affiliation></author><author><name sortKey="Hariharan, Kandasamy" sort="Hariharan, Kandasamy" uniqKey="Hariharan K" first="Kandasamy" last="Hariharan">Kandasamy Hariharan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>IDEC Pharmaceuticals Corporation, San Diego, CA 92121</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Srikumaran, S" sort="Srikumaran, S" uniqKey="Srikumaran S" first="S." last="Srikumaran">S. Srikumaran</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Fair Street and East Campus Loop, Lincoln, NE 68583-0905</wicri:regionArea><placeName><region type="state">Nebraska</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">7–8</biblScope><biblScope unit="page" from="686">686</biblScope><biblScope unit="page" to="694">694</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Allele</term><term>Aspms</term><term>Assay</term><term>Bovine</term><term>Bovine herpesvirus</term><term>Cell epitopes</term><term>Ctls</term><term>Cytotoxic</term><term>Cytotoxicity assay</term><term>Epitope</term><term>Error bars</term><term>Glycoprotein</term><term>Herpesvirus</term><term>Immunization</term><term>Immunol</term><term>Individual peptides</term><term>Lymphocyte</term><term>Lysed</term><term>Lysis</term><term>Maximum release</term><term>Molecule</term><term>Murine</term><term>Native peptides</term><term>Particular class</term><term>Peptide</term><term>Peptide motif</term><term>Peptide motifs</term><term>Recombinant</term><term>Spontaneous release</term><term>Standard deviations</term><term>Syngeneic</term><term>Synthetic peptides</term><term>Target cells</term><term>Vaccine</term><term>Viral</term><term>Viral immunol</term><term>Viral peptides</term><term>Virol</term><term>Zatechka</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Major histocompatibility complex (MHC) class I molecules present endogenously derived viral peptides to CD8+ cytotoxic T-lymphocytes (CTLs). The objective of this study was to identify the H-2Dd- and H-2Kd-restricted CTL epitopes of bovine herpesvirus 1 (BHV-1), based on the allele-specific peptide motifs (ASPMs) of the above class I molecules. Nine sequences conforming to the H-2Dd and H-2Kd ASPMs were identified on BHV-1 proteins, and the respective peptides were synthesized. Five of these peptides exhibited moderate to strong binding to the Dd molecule. CTLs generated by BALB/c mice immunized with BHV-1 proteins emulsified in a suitable adjuvant effectively lysed peptide-pulsed syngeneic targets, indicating that these epitopes were generated in vivo. Mice immunized with these peptides emulsified in a suitable adjuvant also developed anti-BHV-1 CTLs. These CTLs identified three veritable CTL epitopes among the “potential epitopes” synthesized based on the ASPMs. The elucidation of the CTL epitopes of BHV-1 should aid in the development of efficacious vaccines against this virus.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Nebraska</li></region></list><tree><country name="États-Unis"><region name="Nebraska"><name sortKey="Zatechka Jr, Douglas S" sort="Zatechka Jr, Douglas S" uniqKey="Zatechka Jr D" first="Douglas S." last="Zatechka Jr.">Douglas S. Zatechka Jr.</name></region><name sortKey="Hariharan, Kandasamy" sort="Hariharan, Kandasamy" uniqKey="Hariharan K" first="Kandasamy" last="Hariharan">Kandasamy Hariharan</name><name sortKey="Hegde, Nagendra R" sort="Hegde, Nagendra R" uniqKey="Hegde N" first="Nagendra R." last="Hegde">Nagendra R. Hegde</name><name sortKey="Srikumaran, S" sort="Srikumaran, S" uniqKey="Srikumaran S" first="S." last="Srikumaran">S. 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